› 2ikq

X-ray diffraction
2.61Å resolution

Crystal structure of mouse Sts-1 PGM domain in complex with phosphate

Released:
DOI: 10.2210/pdb2ikq/pdb
PDB entry 2ikq coloured by chain, front view.
PDB entry 2ikq coloured by chain, side view.
PDB entry 2ikq coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
A phosphatase activity of Sts-1 contributes to the suppression of TCR signaling.
Mikhailik A, Ford B, Keller J, Chen Y, Nassar N, Carpino N
Mol Cell 27 486-97 (2007)
PMID: 17679096

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183858 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-associated and SH3 domain-containing protein B Chains: A, B, M
Molecule details ›
Chains: A, B, M
Length: 270 amino acids
Theoretical weight: 30.36 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8BGG7 (Residues: 369-638; Coverage: 42%)
Gene names: Sts1, Ubash3b
Sequence domains: Histidine phosphatase superfamily (branch 1)
Structure domains: Phosphoglycerate mutase-like

Ligands and Environments

1 bound ligand:
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: C2
Unit cell:
a: 116.96Å b: 74.671Å c: 101.279Å
α: 90° β: 100.79° γ: 90°
R-values:
R R work R free
0.249 0.248 0.275
Expression system: Escherichia coli

Quick links

Citations

19 review citations

T cell activation.
Smith-Garvin et al. (2009)
18 more

3 mentions without citation

A phosphatase activity of Sts-1 contributes to the suppression of TCR signaling.
Mikhailik et al. (2007)
2 more