› 2iph

X-ray diffraction
1.75Å resolution

X-ray Structure at 1.75 A Resolution of a Norovirus Protease Linked to an Active Site Directed Peptide Inhibitor

Released:
DOI: 10.2210/pdb2iph/pdb
PDB entry 2iph coloured by chain, front view.
PDB entry 2iph coloured by chain, side view.
PDB entry 2iph coloured by chain, top view.
Source organism: Southampton virus (serotype 3)
Primary publication:
A structural study of norovirus 3C protease specificity: binding of a designed active site-directed peptide inhibitor.
Hussey RJ, Coates L, Gill RS, Erskine PT, Coker SF, Mitchell E, Cooper JB, Wood S, Broadbridge R, Clarke IN, Lambden PR, Shoolingin-Jordan PM
Biochemistry 50 240-9 (2011)
PMID: 21128685

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|- and the P1' position is occupied by Gly-|-
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170037 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3C-like protease Chains: A, B
Molecule details ›
Chains: A, B
Length: 181 amino acids
Theoretical weight: 19.31 KDa
Source organism: Southampton virus (serotype 3)
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04544 (Residues: 1100-1280; Coverage: 10%)
Gene name: ORF1
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:
Ligand LGG 2 x LGG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 49.497Å b: 84.106Å c: 121.471Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.221
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Norovirus: targets and tools in antiviral drug discovery.
Rocha-Pereira et al. (2014)
6 more

8 mentions without citation

A structural study of norovirus 3C protease specificity: binding of a designed active site-directed peptide inhibitor.
Hussey et al. (2011)
7 more