2itl

X-ray diffraction
1.65Å resolution

The origin binding domain of the SV40 large T antigen bound to the functional pen palindrome DNA (23 bp)

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-115414 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Large T antigen Chains: A, B
Molecule details ›
Chains: A, B
Length: 133 amino acids
Theoretical weight: 15.38 KDa
Source organism: Macaca mulatta polyomavirus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q98ZP7 (Residues: 131-259; Coverage: 24%)
Sequence domains: Origin of replication binding protein
Structure domains: Replication Protein E1; Chain: A,
24-nt PEN element of the SV40 DNA origin Chain: W
Molecule details ›
Chain: W
Length: 24 nucleotides
Theoretical weight: 7.4 KDa
Source organism: Synthetic construct
Expression system: Not provided
24-nt PEN element of the SV40 DNA origin Chain: C
Molecule details ›
Chain: C
Length: 24 nucleotides
Theoretical weight: 7.36 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: C2
Unit cell:
a: 83.196Å b: 64.096Å c: 79.177Å
α: 90° β: 120.69° γ: 90°
R-values:
R R work R free
0.204 0.201 0.251
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided