› 2iu1

X-ray diffraction
1.8Å resolution

Crystal structure of eIF5 C-terminal domain

Released:
DOI: 10.2210/pdb2iu1/pdb
PDB entry 2iu1 coloured by chain, front view.
PDB entry 2iu1 coloured by chain, side view.
PDB entry 2iu1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of the carboxy-terminal domain of human translation initiation factor eIF5.
Bieniossek C, Schütz P, Bumann M, Limacher A, Uson I, Baumann U
J Mol Biol 360 457-65 (2006)
PMID: 16781736

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157147 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic translation initiation factor 5 Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 24.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P55010 (Residues: 232-431; Coverage: 46%)
Gene name: EIF5
Sequence domains: eIF4-gamma/eIF5/eIF2-epsilon
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 32.137Å b: 70.254Å c: 81.705Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.185 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

The scanning mechanism of eukaryotic translation initiation.
Hinnebusch AG. (2014)
8 more

11 mentions without citation

Macromolecular ab initio phasing enforcing secondary and tertiary structure.
Millán et al. (2015)
10 more