› 2iug

X-ray diffraction
1.89Å resolution

Crystal structure of the PI3-kinase p85 N-terminal SH2 domain

Released:
DOI: 10.2210/pdb2iug/pdb
PDB entry 2iug coloured by chain, front view.
PDB entry 2iug coloured by chain, side view.
PDB entry 2iug coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the PI 3-kinase p85 amino-terminal SH2 domain and its phosphopeptide complexes.
Nolte RT, Eck MJ, Schlessinger J, Shoelson SE, Harrison SC
Nat Struct Biol 3 364-74 (1996)
PMID: 8599763

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151131 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol 3-kinase regulatory subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 120 amino acids
Theoretical weight: 13.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27986 (Residues: 321-440; Coverage: 17%)
Gene names: GRB1, PIK3R1
Sequence domains: SH2 domain
Structure domains: SH2 domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P41212
Unit cell:
a: 57.12Å b: 57.12Å c: 112.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.289
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Phosphoinositide kinases.
Fruman et al. (1998)
13 more

13 mentions without citation

Somatic mutations in PI3Kalpha: structural basis for enzyme activation and drug design.
Gabelli et al. (2010)
12 more