› 2iyi

X-ray diffraction
2.95Å resolution

structure of a light-induced intermediate of the BLUF domain of the rhodobacterial protein AppA

Released:
DOI: 10.2210/pdb2iyi/pdb
PDB entry 2iyi coloured by chain, front view.
PDB entry 2iyi coloured by chain, side view.
PDB entry 2iyi coloured by chain, top view.
Source organism: Cereibacter sphaeroides
Primary publication:
Crystal structures of the AppA BLUF domain photoreceptor provide insights into blue light-mediated signal transduction.
Jung A, Reinstein J, Domratcheva T, Shoeman RL, Schlichting I
J Mol Biol 362 717-32 (2006)
PMID: 16949615

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174619 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BLUF domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 13.92 KDa
Source organism: Cereibacter sphaeroides
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3J677 (Residues: 1-124; Coverage: 28%)
Gene names: RSP_1565, appA
Sequence domains: Sensors of blue-light using FAD
Structure domains: Alpha-Beta Plaits

Ligands and Environments

3 bound ligands:
Ligand FMN 2 x FMN
Ligand DTU 1 x DTU
Ligand DTT 1 x DTT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P6522
Unit cell:
a: 126.4Å b: 126.4Å c: 64.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.188 0.228
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Structural and mechanistic determinants of c-di-GMP signalling.
Schirmer et al. (2009)
16 more

7 mentions without citation

Seeing the light with BLUF proteins.
Park et al. (2017)
6 more