› 2izv

X-ray diffraction
2.55Å resolution

CRYSTAL STRUCTURE OF SOCS-4 IN COMPLEX WITH ELONGIN-B AND ELONGIN-C AT 2.55A RESOLUTION

Released:
DOI: 10.2210/pdb2izv/pdb
PDB entry 2izv coloured by chain, front view.
PDB entry 2izv coloured by chain, side view.
PDB entry 2izv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the SOCS4-ElonginB/C complex reveals a distinct SOCS box interface and the molecular basis for SOCS-dependent EGFR degradation.
Bullock AN, Rodriguez MC, Debreczeni JE, Songyang Z, Knapp S
Structure 15 1493-504 (2007)
PMID: 17997974

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-172264 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Suppressor of cytokine signaling 4 Chain: A
Molecule details ›
Chain: A
Length: 187 amino acids
Theoretical weight: 21.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8WXH5 (Residues: 274-437; Coverage: 37%)
Gene names: SOCS4, SOCS7
Sequence domains:
Structure domains: SH2 domain
Elongin-B Chain: B
Molecule details ›
Chain: B
Length: 118 amino acids
Theoretical weight: 13.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15370 (Residues: 1-118; Coverage: 100%)
Gene names: ELOB, TCEB2
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Elongin-C Chain: C
Molecule details ›
Chain: C
Length: 97 amino acids
Theoretical weight: 10.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15369 (Residues: 17-112; Coverage: 86%)
Gene names: ELOC, TCEB1
Sequence domains: Skp1 family, tetramerisation domain
Structure domains: Potassium Channel Kv1.1; Chain A

Ligands and Environments

3 bound ligands:
Ligand NA 1 x NA
Ligand EDO 1 x EDO
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: R3
Unit cell:
a: 154.77Å b: 154.77Å c: 67.909Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.172 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

SOCS regulation of the JAK/STAT signalling pathway.
Croker et al. (2008)
26 more

8 mentions without citation

Functional and structural characteristics of bacterial proteins that bind host cytokines.
Högbom et al. (2017)
7 more