› 2izz

X-ray diffraction
1.95Å resolution

Crystal structure of human pyrroline-5-carboxylate reductase

Released:
DOI: 10.2210/pdb2izz/pdb
PDB entry 2izz coloured by chain, front view.
PDB entry 2izz coloured by chain, side view.
PDB entry 2izz coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Pike ACW, Guo K, Kavanagh K, Pilka ES, Berridge G, Colebrook S, Bray J, Salah E, Savitsky P, Papagrigoriou E, Turnbull AP, von Delft F, Arrowsmith C, Edwards A, Weigelt J, Sundstrom M, Oppermann U

Function and Biology Details

Reaction catalysed: 
L-proline + NAD(P)(+) = 1-pyrroline-5-carboxylate + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyrroline-5-carboxylate reductase 1, mitochondrial Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 322 amino acids
Theoretical weight: 34.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32322 (Residues: 1-300; Coverage: 94%)
Gene name: PYCR1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 5 x NAD
1 bound ligand:
Ligand EDO 7 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 110.453Å b: 177.944Å c: 87.916Å
α: 90° β: 106.7° γ: 90°
R-values:
R R work R free
0.167 0.166 0.19
Expression system: Escherichia coli BL21(DE3)

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