› 2j0l

X-ray diffraction
2.3Å resolution

Crystal structure of a the active conformation of the kinase domain of focal adhesion kinase with a phosphorylated activation loop.

Released:
DOI: 10.2210/pdb2j0l/pdb
PDB entry 2j0l coloured by chain, front view.
PDB entry 2j0l coloured by chain, side view.
PDB entry 2j0l coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Structural basis for the autoinhibition of focal adhesion kinase.
Lietha D, Cai X, Ceccarelli DF, Li Y, Schaller MD, Eck MJ
Cell 129 1177-87 (2007)
PMID: 17574028

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169484 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Focal adhesion kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.92 KDa
Source organism: Gallus gallus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q00944 (Residues: 411-686; Coverage: 26%)
Gene names: FAK, FAK1, PTK2
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 1 x SO4
Ligand MG 1 x MG
Ligand ANP 1 x ANP
1 modified residue:
Ligand PTR 2 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 44.312Å b: 71.809Å c: 86.939Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.214 0.263
Expression system: Trichoplusia ni

Quick links

Citations

79 review citations

Cell adhesion: integrating cytoskeletal dynamics and cellular tension.
Parsons et al. (2010)
78 more

8 mentions without citation

FAK Structure and Regulation by Membrane Interactions and Force in Focal Adhesions.
Tapial Martínez et al. (2020)
7 more