› 2j0q

X-ray diffraction
3.2Å resolution

The crystal structure of the Exon Junction Complex at 3.2 A resolution

Released:
DOI: 10.2210/pdb2j0q/pdb
PDB entry 2j0q coloured by chain, front view.
PDB entry 2j0q coloured by chain, side view.
PDB entry 2j0q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of the exon junction complex reveals how it maintains a stable grip on mRNA.
Bono F, Ebert J, Lorentzen E, Conti E
Cell 126 713-25 (2006)
PMID: 16923391

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127367 (preferred)
Entry contents:
4 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (5 distinct):
Eukaryotic initiation factor 4A-III Chains: A, B
Molecule details ›
Chains: A, B
Length: 410 amino acids
Theoretical weight: 46.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38919 (Residues: 2-411; Coverage: 100%)
Gene names: DDX48, EIF4A3, KIAA0111
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Protein mago nashi homolog Chains: C, F
Molecule details ›
Chains: C, F
Length: 146 amino acids
Theoretical weight: 17.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61326 (Residues: 1-146; Coverage: 100%)
Gene names: MAGOH, MAGOHA
Sequence domains: Mago nashi protein
Structure domains: Mago nashi
RNA-binding protein 8A Chains: D, G
Molecule details ›
Chains: D, G
Length: 109 amino acids
Theoretical weight: 12.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y5S9 (Residues: 66-174; Coverage: 63%)
Gene names: HSPC114, MDS014, RBM8, RBM8A
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
Protein CASC3 Chains: I, T
Molecule details ›
Chains: I, T
Length: 150 amino acids
Theoretical weight: 17.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15234 (Residues: 137-286; Coverage: 21%)
Gene names: CASC3, MLN51
Sequence domains: CASC3/Barentsz eIF4AIII binding
5'-R(*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)-3' Chains: E, H
Molecule details ›
Chains: E, H
Length: 15 nucleotides
Theoretical weight: 4.55 KDa

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ANP 2 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 69.15Å b: 161.24Å c: 193.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.232 0.277
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

76 review citations

RNA-binding proteins and post-transcriptional gene regulation.
Glisovic et al. (2008)
75 more

5 mentions without citation

Towards deciphering the principles underlying an mRNA recognition code.
Serganov et al. (2008)
4 more