› 2j4k

X-ray diffraction
2.2Å resolution

Crystal structure of uridylate kinase from Sulfolobus solfataricus in complex with UMP to 2.2 Angstrom resolution

Released:
DOI: 10.2210/pdb2j4k/pdb
PDB entry 2j4k coloured by chain, front view.
PDB entry 2j4k coloured by chain, side view.
PDB entry 2j4k coloured by chain, top view.
Source organism: Saccharolobus solfataricus P2
Primary publication:
Structural and enzymatic investigation of the Sulfolobus solfataricus uridylate kinase shows competitive UTP inhibition and the lack of GTP stimulation.
Jensen KS, Johansson E, Jensen KF
Biochemistry 46 2745-57 (2007)
PMID: 17297917

Function and Biology Details

Reaction catalysed: 
ATP + UMP = ADP + UDP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189071 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridylate kinase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 226 amino acids
Theoretical weight: 25.02 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97ZE2 (Residues: 2-227; Coverage: 100%)
Gene names: SSO0976, pyrH
Sequence domains: Amino acid kinase family
Structure domains: Acetylglutamate kinase-like

Ligands and Environments

3 bound ligands:
Ligand U5P 6 x U5P
Ligand CD 18 x CD
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P21
Unit cell:
a: 74.802Å b: 136.043Å c: 77.938Å
α: 90° β: 113.17° γ: 90°
R-values:
R R work R free
0.219 0.219 0.251
Expression system: Escherichia coli

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Citations

13 citation in other articles

The structural basis of ATP as an allosteric modulator.
Lu et al. (2014)
12 more