› 2j5t

X-ray diffraction
2.9Å resolution

Glutamate 5-kinase from Escherichia coli complexed with glutamate

Released:
DOI: 10.2210/pdb2j5t/pdb
PDB entry 2j5t coloured by chain, front view.
PDB entry 2j5t coloured by chain, side view.
PDB entry 2j5t coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A novel two-domain architecture within the amino acid kinase enzyme family revealed by the crystal structure of Escherichia coli glutamate 5-kinase.
Marco-Marín C, Gil-Ortiz F, Pérez-Arellano I, Cervera J, Fita I, Rubio V
J Mol Biol 367 1431-46 (2007)
PMID: 17321544

Function and Biology Details

Reaction catalysed: 
ATP + L-glutamate = ADP + L-glutamate 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141574 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate 5-kinase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 367 amino acids
Theoretical weight: 39.09 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A7B5 (Residues: 1-367; Coverage: 100%)
Gene names: JW0232, b0242, proB
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand GLU 12 x GLU
Ligand SO4 8 x SO4
Ligand CL 20 x CL
Ligand MG 8 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 96.302Å b: 124.108Å c: 144.927Å
α: 90° β: 93.96° γ: 90°
R-values:
R R work R free
0.199 0.197 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Proline mechanisms of stress survival.
Liang et al. (2013)
7 more

5 mentions without citation

Pyrroline-5-carboxylate synthase and proline biosynthesis: from osmotolerance to rare metabolic disease.
Pérez-Arellano et al. (2010)
4 more