› 2j7t

X-ray diffraction
2Å resolution

Crystal structure of human serine threonine kinase-10 bound to SU11274

Released:
DOI: 10.2210/pdb2j7t/pdb
PDB entry 2j7t coloured by chain, front view.
PDB entry 2j7t coloured by chain, side view.
PDB entry 2j7t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Activation segment dimerization: a mechanism for kinase autophosphorylation of non-consensus sites.
Pike AC, Rellos P, Niesen FH, Turnbull A, Oliver AW, Parker SA, Turk BE, Pearl LH, Knapp S
EMBO J 27 704-14 (2008)
PMID: 18239682

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131760 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase 10 Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 34.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O94804 (Residues: 18-317; Coverage: 31%)
Gene names: LOK, STK10
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 7 x CA
Ligand ACT 1 x ACT
Ligand 274 1 x 274
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: I222
Unit cell:
a: 49.192Å b: 112.96Å c: 133.817Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.204 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

The structural basis for control of eukaryotic protein kinases.
Endicott et al. (2012)
16 more

10 mentions without citation

Substrate and docking interactions in serine/threonine protein kinases.
Goldsmith et al. (2007)
9 more