› 2j90

X-ray diffraction
2Å resolution

Crystal structure of human ZIP kinase in complex with a tetracyclic pyridone inhibitor (Pyridone 6)

Released:
DOI: 10.2210/pdb2j90/pdb
PDB entry 2j90 coloured by chain, front view.
PDB entry 2j90 coloured by chain, side view.
PDB entry 2j90 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Activation segment dimerization: a mechanism for kinase autophosphorylation of non-consensus sites.
Pike AC, Rellos P, Niesen FH, Turnbull A, Oliver AW, Parker SA, Turk BE, Pearl LH, Knapp S
EMBO J 27 704-14 (2008)
PMID: 18239682

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128886 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Death-associated protein kinase 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 304 amino acids
Theoretical weight: 35.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43293 (Residues: 2-289; Coverage: 63%)
Gene names: DAPK3, ZIPK
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand IZA 2 x IZA
Ligand CL 2 x CL
Ligand EDO 3 x EDO
Ligand PO4 1 x PO4
2 modified residues:
Ligand SEP 2 x SEP
Ligand TPO 2 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P43212
Unit cell:
a: 95.765Å b: 95.765Å c: 150.941Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.222
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

The structural basis for control of eukaryotic protein kinases.
Endicott et al. (2012)
16 more

7 mentions without citation

Substrate and docking interactions in serine/threonine protein kinases.
Goldsmith et al. (2007)
6 more