› 2j91

X-ray diffraction
1.8Å resolution

Crystal structure of Human Adenylosuccinate Lyase in complex with AMP

Released:
DOI: 10.2210/pdb2j91/pdb
PDB entry 2j91 coloured by chain, front view.
PDB entry 2j91 coloured by chain, side view.
PDB entry 2j91 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Stenmark P, Moche M, Arrowsmith C, Berglund H, Busam R, Collins R, Edwards A, Ericsson UB, Flodin S, Flores A, Graslund S, Hammarstrom M, Hallberg BM, Holmberg Schiavone L, Hogbom M, Johansson I, Karlberg T, Kosinska U, Kotenyova T, Magnusdottir A, Nilsson ME, Nilsson-Ehle P, Nyman T, Ogg D, Persson C, Sagemark J, Sundstrom M, Uppenberg J, Uppsten M, Thorsell AG, van Den Berg S, Wallden K, Weigelt J, Nordlund P

Function and Biology Details

Reaction catalysed: 
(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151766 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate lyase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 503 amino acids
Theoretical weight: 57.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30566 (Residues: 1-481; Coverage: 99%)
Gene names: ADSL, AMPS
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand AMP 4 x AMP
Ligand CL 4 x CL
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P212121
Unit cell:
a: 85.37Å b: 104.342Å c: 213.396Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.157 0.199
Expression system: Escherichia coli BL21(DE3)

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