› 2j9n

X-ray diffraction
1.5Å resolution

Robotically harvested Trypsin complexed with Benzamidine containing polypeptide mediated crystal contacts

Released:
DOI: 10.2210/pdb2j9n/pdb
PDB entry 2j9n coloured by chain, front view.
PDB entry 2j9n coloured by chain, side view.
PDB entry 2j9n coloured by chain, top view.
Source organisms:
Primary publication:
Operator-assisted harvesting of protein crystals using a universal micromanipulation robot.
Viola R, Carman P, Walsh J, Miller E, Benning M, Frankel D, McPherson A, Cudney B, Rupp B
J Appl Crystallogr 40 539-545 (2007)
PMID: 19461845

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133350 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Serine protease 1 Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 23.32 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00760 (Residues: 24-246; Coverage: 97%)
Gene names: PRSS1, TRP1, TRY1, TRYP1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
UNKNOWN PEPTIDE Chain: B
Molecule details ›
Chain: B
Length: 15 amino acids
Theoretical weight: 1.29 KDa
Source organism: synthetic construct
Expression system: Not provided
UNKNOWN PEPTIDE Chain: C
Molecule details ›
Chain: C
Length: 3 amino acids
Theoretical weight: 316 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand CA 1 x CA
Ligand NA 1 x NA
Ligand BEN 1 x BEN
Ligand IPH 1 x IPH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P212121
Unit cell:
a: 53.924Å b: 56.695Å c: 66.054Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.155 0.154 0.176
Expression system: Not provided

Quick links

Citations

3 review citations

A historical perspective on protein crystallization from 1840 to the present day.
Giegé R. (2013)
2 more

4 mentions without citation

The solvent component of macromolecular crystals.
Weichenberger et al. (2015)
3 more