› 2jbv

X-ray diffraction
1.86Å resolution

Crystal structure of choline oxidase reveals insights into the catalytic mechanism

Released:
DOI: 10.2210/pdb2jbv/pdb
PDB entry 2jbv coloured by chain, front view.
PDB entry 2jbv coloured by chain, side view.
PDB entry 2jbv coloured by chain, top view.
Source organism: Arthrobacter globiformis
Primary publication:
Role of Glu312 in binding and positioning of the substrate for the hydride transfer reaction in choline oxidase.
Quaye O, Lountos GT, Fan F, Orville AM, Gadda G
Biochemistry 47 243-56 (2008)
PMID: 18072756

Function and Biology Details

Reaction catalysed: 
(1a) choline + O(2) = betaine aldehyde + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182177 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Choline oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 546 amino acids
Theoretical weight: 59.9 KDa
Source organism: Arthrobacter globiformis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7X2H8 (Residues: 1-546; Coverage: 100%)
Gene name: codA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAO 2 x FAO
2 bound ligands:
Ligand DMS 2 x DMS
Ligand UNX 4 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43212
Unit cell:
a: 84.355Å b: 84.355Å c: 343.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.212
Expression system: Escherichia coli

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Citations

3 review citations

Fungal aryl-alcohol oxidase: a peroxide-producing flavoenzyme involved in lignin degradation.
Hernández-Ortega et al. (2012)
2 more

11 mentions without citation

Complete genome sequence and metabolic potential of the quinaldine-degrading bacterium Arthrobacter sp. Rue61a.
Niewerth et al. (2012)
10 more