› 2je7

X-ray diffraction
1.65Å resolution

Crystal structure of recombinant Dioclea guianensis lectin S131H complexed with 5-bromo-4-chloro-3-indolyl-a-D-mannose

Released:
DOI: 10.2210/pdb2je7/pdb
PDB entry 2je7 coloured by chain, front view.
PDB entry 2je7 coloured by chain, side view.
PDB entry 2je7 coloured by chain, top view.
Source organism: Dioclea guianensis
Primary publication:
Insights into the structural basis of the pH-dependent dimer-tetramer equilibrium through crystallographic analysis of recombinant Diocleinae lectins.
Nagano CS, Calvete JJ, Barettino D, Pérez A, Cavada BS, Sanz L
Biochem J 409 417-28 (2008)
PMID: 17937659

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160543 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lectin alpha chain Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 25.7 KDa
Source organism: Dioclea guianensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P81637 (Residues: 1-237; Coverage: 100%)
Sequence domains: Legume lectin domain
Structure domains: Jelly Rolls

Ligands and Environments

3 bound ligands:
Ligand MN 1 x MN
Ligand CA 1 x CA
Ligand XMM 1 x XMM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: I222
Unit cell:
a: 65.661Å b: 88.014Å c: 91.536Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.201
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

ConA-Like Lectins: High Similarity Proteins as Models to Study Structure/Biological Activities Relationships.
Cavada et al. (2018)
1 more

1 mention without citation

Sequence-Based Prediction of Metamorphic Behavior in Proteins.
Chen et al. (2020)