› 2jg4

X-ray diffraction
2.8Å resolution

Substrate-free IDE structure in its closed conformation

Released:
DOI: 10.2210/pdb2jg4/pdb
PDB entry 2jg4 coloured by chain, front view.
PDB entry 2jg4 coloured by chain, side view.
PDB entry 2jg4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of substrate-free human insulin-degrading enzyme (IDE) and biophysical analysis of ATP-induced conformational switch of IDE.
Im H, Manolopoulou M, Malito E, Shen Y, Zhao J, Neant-Fery M, Sun CY, Meredith SC, Sisodia SS, Leissring MA, Tang WJ
J Biol Chem 282 25453-63 (2007)
PMID: 17613531

Function and Biology Details

Reaction catalysed: 
Degradation of insulin, glucagon and other polypeptides. No action on proteins.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147037 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Insulin-degrading enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 990 amino acids
Theoretical weight: 114.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14735 (Residues: 42-1019; Coverage: 96%)
Gene name: IDE
Sequence domains:
Structure domains: Metalloenzyme, LuxS/M16 peptidase-like

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand DIO 4 x DIO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P65
Unit cell:
a: 263.211Å b: 263.211Å c: 90.408Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.182 0.227
Expression system: Escherichia coli

Quick links

Citations

17 review citations

European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS).
Egea et al. (2017)
16 more

12 mentions without citation

Amyloid beta-degrading cryptidases: insulin degrading enzyme, presequence peptidase, and neprilysin.
Malito et al. (2008)
11 more