› 2jg7

X-ray diffraction
2.83Å resolution

Crystal structure of Seabream Antiquitin and Elucidation of its substrate specificity

Released:
DOI: 10.2210/pdb2jg7/pdb
PDB entry 2jg7 coloured by chain, front view.
PDB entry 2jg7 coloured by chain, side view.
PDB entry 2jg7 coloured by chain, top view.
Source organism: Acanthopagrus schlegelii
Primary publication:
The crystal structure of seabream antiquitin reveals the structural basis of its substrate specificity.
Tang WK, Wong KB, Lam YM, Cha SS, Cheng CH, Fong WP
FEBS Lett 582 3090-6 (2008)
PMID: 18694748

Function and Biology Details

Reaction catalysed: 
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175638 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
aldehyde dehydrogenase (NAD(+)) Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 510 amino acids
Theoretical weight: 55.22 KDa
Source organism: Acanthopagrus schlegelii
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q4KTQ7 (Residues: 2-511; Coverage: 100%)
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 8 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS
Spacegroup: P1
Unit cell:
a: 88.16Å b: 111.02Å c: 113.64Å
α: 90.5° β: 98.1° γ: 111.59°
R-values:
R R work R free
0.211 0.21 0.228
Expression system: Escherichia coli BL21

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Citations

1 review citation

Pyridoxine dependent epilepsy and antiquitin deficiency: clinical and molecular characteristics and recommendations for diagnosis, treatment and follow-up.
Stockler et al. (2011)
13 other articles