› 2jkx

X-ray diffraction
1.84Å resolution

Galactose oxidase. MatGO. Copper free, expressed in Pichia Pastoris.

Released:
DOI: 10.2210/pdb2jkx/pdb
PDB entry 2jkx coloured by chain, front view.
PDB entry 2jkx coloured by chain, side view.
PDB entry 2jkx coloured by chain, top view.
Source organism: Fusarium graminearum
Primary publication:
Enhanced fructose oxidase activity in a galactose oxidase variant.
Deacon SE, Mahmoud K, Spooner RK, Firbank SJ, Knowles PF, Phillips SE, McPherson MJ
Chembiochem 5 972-9 (2004)
PMID: 15239055

Function and Biology Details

Reaction catalysed: 
D-galactose + O(2) = D-galacto-hexodialdose + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143550 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactose oxidase Chain: A
Molecule details ›
Chain: A
Length: 639 amino acids
Theoretical weight: 68.58 KDa
Source organism: Fusarium graminearum
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P0CS93 (Residues: 42-680; Coverage: 97%)
Gene name: GAOA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: C2
Unit cell:
a: 96.95Å b: 88.52Å c: 85.35Å
α: 90° β: 117.24° γ: 90°
R-values:
R R work R free
0.167 0.163 0.201
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

The substrate tolerance of alcohol oxidases.
Pickl et al. (2015)
17 other articles

1 mention without citation

Copper active sites in biology.
Solomon et al. (2014)