› 2jl9

X-ray diffraction
3.2Å resolution

Structural explanation for the role of Mn in the activity of phi6 RNA- dependent RNA polymerase

Released:
DOI: 10.2210/pdb2jl9/pdb
PDB entry 2jl9 coloured by chain, front view.
PDB entry 2jl9 coloured by chain, side view.
PDB entry 2jl9 coloured by chain, top view.
Source organism: Pseudomonas virus phi6
Primary publication:
Structural explanation for the role of Mn2+ in the activity of phi6 RNA-dependent RNA polymerase.
Poranen MM, Salgado PS, Koivunen MR, Wright S, Bamford DH, Stuart DI, Grimes JM
Nucleic Acids Res 36 6633-44 (2008)
PMID: 18940872

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145660 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 665 amino acids
Theoretical weight: 75.03 KDa
Source organism: Pseudomonas virus phi6
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P11124 (Residues: 1-665; Coverage: 100%)
Gene name: P2
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P21
Unit cell:
a: 106.519Å b: 91.599Å c: 142.705Å
α: 90° β: 101.64° γ: 90°
R-values:
R R work R free
0.218 0.215 0.272
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Global genomics and proteomics approaches to identify host factors as targets to induce resistance against Tomato bushy stunt virus.
Nagy et al. (2010)
4 more

2 mentions without citation

Cystoviral RNA-directed RNA polymerases: Regulation of RNA synthesis on multiple time and length scales.
Alphonse et al. (2017)
1 more