› 2jmo

Solution NMR

IBR domain of Human Parkin

Released:
DOI: 10.2210/pdb2jmo/pdb
PDB entry 2jmo coloured by chain, ensemble of 20 models, front view.
PDB entry 2jmo coloured by chain, ensemble of 20 models, side view.
PDB entry 2jmo coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the Parkin in-between-ring domain provides insights for E3-ligase dysfunction in autosomal recessive Parkinson's disease.
Beasley SA, Hristova VA, Shaw GS
Proc Natl Acad Sci U S A 104 3095-100 (2007)
PMID: 17360614

Function and Biology Details

Reaction catalysed: 
(1a) [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RBR-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RBR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129926 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase parkin Chain: A
Molecule details ›
Chain: A
Length: 80 amino acids
Theoretical weight: 8.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60260 (Residues: 308-384; Coverage: 17%)
Gene names: PARK2, PRKN
Structure domains: N-terminal domain of TfIIb

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing, torsion angle dynamics
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Structural insights into the catalysis and regulation of E3 ubiquitin ligases.
Buetow et al. (2016)
12 more

8 mentions without citation

RBR E3 ubiquitin ligases: new structures, new insights, new questions.
Spratt et al. (2014)
7 more