Function and Biology Details
Reaction catalysed:
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Sequence domains:
Structure analysis Details
Assembly composition:
monomeric (preferred)
Assembly name:
E3 ubiquitin-protein ligase SMURF2 (preferred)
PDBe Complex ID:
PDB-CPX-190727 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase SMURF2
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 14.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: C2 domain
Structure domains: C2 domain
Length: 131 amino acids
Theoretical weight: 14.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
Q9HAU4 (Residues: 10-140; Coverage: 18%)
Sequence domains: C2 domain
Structure domains: C2 domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Chemical shift assignment:
80%
Refinement method:
simulated annealing, torsion angle dynamics
Expression system: Escherichia coli
