› 2jri

Solution NMR

Solution structure of the Josephin domain of Ataxin-3 in complex with ubiquitin molecule.

Released:
DOI: 10.2210/pdb2jri/pdb
PDB entry 2jri coloured by chain, ensemble of 20 models, front view.
PDB entry 2jri coloured by chain, ensemble of 20 models, side view.
PDB entry 2jri coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Entry authors: Nicastro G, Masino L, Esposito V, Menon R, Pastore A

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143422 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ataxin-3 Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 21.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54252 (Residues: 1-182; Coverage: 50%)
Gene names: ATX3, ATXN3, MJD, MJD1, SCA3
Sequence domains: Josephin
Structure domains:
Ubiquitin Chains: B, C
Molecule details ›
Chains: B, C
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)

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