› 2jtz

Solution NMR

Solution structure and chemical shift assignments of the F104-to-5-flurotryptophan mutant of cardiac troponin C

Released:
DOI: 10.2210/pdb2jtz/pdb
PDB entry 2jtz coloured by chain, ensemble of 10 models, front view.
PDB entry 2jtz coloured by chain, ensemble of 10 models, side view.
PDB entry 2jtz coloured by chain, ensemble of 10 models, top view.
Source organism: Homo sapiens
Primary publication:
Effects of Phe-to-Trp mutation and fluorotryptophan incorporation on the solution structure of cardiac troponin C, and analysis of its suitability as a potential probe for in situ NMR studies.
Wang X, Mercier P, Letourneau PJ, Sykes BD
Protein Sci 14 2447-60 (2005)
PMID: 16131667

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159088 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Troponin C, slow skeletal and cardiac muscles Chain: A
Molecule details ›
Chain: A
Length: 161 amino acids
Theoretical weight: 18.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63316 (Residues: 1-161; Coverage: 100%)
Gene names: TNNC, TNNC1
Sequence domains:
Structure domains: EF-hand

Ligands and Environments

No bound ligands
1 modified residue:
Ligand FTR 1 x FTR

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

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Citations

1 review citation

Tinker, Tailor, Soldier, Spy: The Diverse Roles That Fluorine Can Play within Amino Acid Side Chains.
Miles et al. (2023)
11 other articles