Function and Biology Details
Reaction catalysed:
Propanoyl-CoA + 6 (2S)-methylmalonyl-CoA + 6 NADPH = 6-deoxyerythronolide B + 7 CoA + 6 CO(2) + H(2)O + 6 NADP(+)
Biochemical function:
Biological process:
- not assigned
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169837 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-deoxyerythronolide-B synthase EryA1, modules 1 and 2
Molecule details ›
Chain: A
Length: 95 amino acids
Theoretical weight: 10.16 KDa
Source organism: Saccharopolyspora erythraea
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Phosphopantetheine attachment site
Structure domains: ACP-like
Length: 95 amino acids
Theoretical weight: 10.16 KDa
Source organism: Saccharopolyspora erythraea
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q03131 (Residues: 3318-3408; Coverage: 3%)
Sequence domains: Phosphopantetheine attachment site
Structure domains: ACP-like
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Refinement method:
simulated annealing, torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)
