› 2k07

Solution NMR

Solution NMR structure of human E2-like ubiquitin-fold modifier conjugating enzyme 1 (UFC1). Northeast Structural Genomics Consortium target HR41

Released:
DOI: 10.2210/pdb2k07/pdb
PDB entry 2k07 coloured by chain, ensemble of 20 models, front view.
PDB entry 2k07 coloured by chain, ensemble of 20 models, side view.
PDB entry 2k07 coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
NMR and X-RAY structures of human E2-like ubiquitin-fold modifier conjugating enzyme 1 (UFC1) reveal structural and functional conservation in the metazoan UFM1-UBA5-UFC1 ubiquination pathway.
Liu G, Forouhar F, Eletsky A, Atreya HS, Aramini JM, Xiao R, Huang YJ, Abashidze M, Seetharaman J, Liu J, Rost B, Acton T, Montelione GT, Hunt JF, Szyperski T
J Struct Funct Genomics 10 127-36 (2009)
PMID: 19101823

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-fold modifier-conjugating enzyme 1 Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 20.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y3C8 (Residues: 1-167; Coverage: 100%)
Gene names: CGI-126, HSPC155, UFC1
Sequence domains: Ubiquitin-fold modifier-conjugating enzyme 1
Structure domains: Ubiquitin Conjugating Enzyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: molecular dynamics, simulated annealing, distance geometry, torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

The UFMylation System in Proteostasis and Beyond.
Gerakis et al. (2019)
7 more

3 mentions without citation

A community resource of experimental data for NMR / X-ray crystal structure pairs.
Everett et al. (2016)
2 more