› 2k4d

Solution NMR

E2-c-Cbl recognition is necessary but not sufficient for ubiquitination activity

Released:
DOI: 10.2210/pdb2k4d/pdb
PDB entry 2k4d coloured by chain, ensemble of 20 models, front view.
PDB entry 2k4d coloured by chain, ensemble of 20 models, side view.
PDB entry 2k4d coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
E2-c-Cbl recognition is necessary but not sufficient for ubiquitination activity.
Huang A, de Jong RN, Wienk H, Winkler GS, Timmers HT, Boelens R
J Mol Biol 385 507-19 (2009)
PMID: 18996392

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149756 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase CBL Chain: A
Molecule details ›
Chain: A
Length: 83 amino acids
Theoretical weight: 9.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P22681 (Residues: 358-437; Coverage: 9%)
Gene names: CBL, CBL2, RNF55
Sequence domains: Zinc finger, C3HC4 type (RING finger)
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 95%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

7 review citations

RING domain E3 ubiquitin ligases.
Deshaies et al. (2009)
6 more