› 2kdc

Solution NMR

NMR Solution Structure of E. coli diacylglycerol kinase (DAGK) in DPC micelles

Released:
DOI: 10.2210/pdb2kdc/pdb
PDB entry 2kdc coloured by chain, ensemble of 16 models, front view.
PDB entry 2kdc coloured by chain, ensemble of 16 models, side view.
PDB entry 2kdc coloured by chain, ensemble of 16 models, top view.
Source organism: Escherichia coli K-12
Primary publication:
Solution nuclear magnetic resonance structure of membrane-integral diacylglycerol kinase.
Van Horn WD, Kim HJ, Ellis CD, Hadziselimovic A, Sulistijo ES, Karra MD, Tian C, Sönnichsen FD, Sanders CR
Science 324 1726-9 (2009)
PMID: 19556511

Function and Biology Details

Reaction catalysed: 
ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142184 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diacylglycerol kinase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 121 amino acids
Theoretical weight: 13.12 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABN1 (Residues: 2-122; Coverage: 99%)
Gene names: JW4002, b4042, dgk, dgkA
Sequence domains: Prokaryotic diacylglycerol kinase
Structure domains: Diacylglycerol kinase (DAGK) domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

34 review citations

Bacterial lipids: metabolism and membrane homeostasis.
Parsons et al. (2013)
33 more

18 mentions without citation

Influences of membrane mimetic environments on membrane protein structures.
Zhou et al. (2013)
17 more