› 2kdz

Solution NMR

Structure of the R2R3 DNA binding domain of MYB1 protein from protozoan parasite trichomonas vaginalis in complex with MRE-1/MRE-2R DNA

Released:
DOI: 10.2210/pdb2kdz/pdb
PDB entry 2kdz coloured by chain, ensemble of 10 models, front view.
PDB entry 2kdz coloured by chain, ensemble of 10 models, side view.
PDB entry 2kdz coloured by chain, ensemble of 10 models, top view.
Source organism: Trichomonas vaginalis
Primary publication:
NMR structural analysis of DNA recognition by a novel Myb1 DNA-binding domain in the protozoan parasite Trichomonas vaginalis.
Lou YC, Wei SY, Rajasekaran M, Chou CC, Hsu HM, Tai JH, Chen C
Nucleic Acids Res 37 2381-94 (2009)
PMID: 19246540

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115468 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
MYB24 Chain: A
Molecule details ›
Chain: A
Length: 107 amino acids
Theoretical weight: 13.22 KDa
Source organism: Trichomonas vaginalis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58HP2 (Residues: 35-141; Coverage: 52%)
Sequence domains: Myb-like DNA-binding domain
Structure domains: Homeodomain-like
5'-D(*AP*AP*GP*AP*TP*AP*AP*CP*GP*AP*TP*AP*TP*TP*TP*A)-3' Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.93 KDa
Source organism: Trichomonas vaginalis
Expression system: Not provided
5'-D(*TP*AP*AP*AP*TP*AP*TP*CP*GP*TP*TP*AP*TP*CP*TP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.86 KDa
Source organism: Trichomonas vaginalis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing, WATER REFINEMENT
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Regulation of gene expression in protozoa parasites.
Gomez et al. (2010)
1 more

3 mentions without citation

Analysis of the DNA-Binding Activities of the Arabidopsis R2R3-MYB Transcription Factor Family by One-Hybrid Experiments in Yeast.
Kelemen et al. (2015)
2 more