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Solution NMR

Solution NMR structure of human protein HS00059, cytochrome-b5-like domain of the HERC2 E3 ligase. Northeast structural genomics consortium (NESG) target ht98a

Released:
DOI: 10.2210/pdb2keo/pdb
PDB entry 2keo coloured by chain, ensemble of 20 models, front view.
PDB entry 2keo coloured by chain, ensemble of 20 models, side view.
PDB entry 2keo coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Entry authors: Lemak A, Gutmanas A, Fares C, Quyang H, Li Y, Montelione G, Arrowsmith C, Dhe-Paganon S, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131992 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase HERC2 Chain: A
Molecule details ›
Chain: A
Length: 112 amino acids
Theoretical weight: 12.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95714 (Residues: 1203-1296; Coverage: 2%)
Gene name: HERC2
Sequence domains: Cytochrome b5-like Heme/Steroid binding domain
Structure domains: Cytochrome b5-like heme/steroid binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: restrained molecular dynamics in water bath
Expression system: Escherichia coli

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