› 2khx

Solution NMR

Drosha double-stranded RNA binding motif

Released:
DOI: 10.2210/pdb2khx/pdb
PDB entry 2khx coloured by chain, ensemble of 10 models, front view.
PDB entry 2khx coloured by chain, ensemble of 10 models, side view.
PDB entry 2khx coloured by chain, ensemble of 10 models, top view.
Source organism: Homo sapiens
Primary publication:
Solution structure of the Drosha double-stranded RNA-binding domain.
Mueller GA, Miller MT, DeRose EF, Ghosh M, London RE, Hall TMT
Silence 1 2 (2010)
PMID: 20226070

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192567 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease 3 Chain: A
Molecule details ›
Chain: A
Length: 85 amino acids
Theoretical weight: 9.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NRR4 (Residues: 1259-1337; Coverage: 6%)
Gene names: DROSHA, RN3, RNASE3L, RNASEN
Sequence domains: Double-stranded RNA binding motif
Structure domains: Double Stranded RNA Binding Domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 75%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Molecular mechanisms of RNA interference.
Wilson et al. (2013)
5 more

6 mentions without citation

RNA recognition by double-stranded RNA binding domains: a matter of shape and sequence.
Masliah et al. (2013)
5 more