› 2kyu

Solution NMR

The solution structure of the PHD3 finger of MLL

Released:
DOI: 10.2210/pdb2kyu/pdb
PDB entry 2kyu coloured by chain, ensemble of 10 models, front view.
PDB entry 2kyu coloured by chain, ensemble of 10 models, side view.
PDB entry 2kyu coloured by chain, ensemble of 10 models, top view.
Source organism: Homo sapiens
Primary publication:
The PHD3 domain of MLL acts as a CYP33-regulated switch between MLL-mediated activation and repression .
Park S, Osmers U, Raman G, Schwantes RH, Diaz MO, Bushweller JH
Biochemistry 49 6576-86 (2010)
PMID: 20677832

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + a [histone H3]-L-lysine(4) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(4)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169856 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MLL cleavage product N320 Chain: A
Molecule details ›
Chain: A
Length: 67 amino acids
Theoretical weight: 7.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03164 (Residues: 1564-1628; Coverage: 2%)
Gene names: ALL1, CXXC7, HRX, HTRX, KMT2A, MLL, MLL1, TRX1
Sequence domains: PHD-finger
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 92%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Handpicking epigenetic marks with PHD fingers.
Musselman et al. (2011)
21 more

4 mentions without citation

Modulation of epigenetic targets for anticancer therapy: clinicopathological relevance, structural data and drug discovery perspectives.
Andreoli et al. (2013)
3 more