› 2l1b

Solution NMR

Solution NMR structure of the chromobox protein Cbx7 with H3K27me3

Released:
DOI: 10.2210/pdb2l1b/pdb
PDB entry 2l1b coloured by chain, ensemble of 20 models, front view.
PDB entry 2l1b coloured by chain, ensemble of 20 models, side view.
PDB entry 2l1b coloured by chain, ensemble of 20 models, top view.
Source organisms:
Primary publication:
Recognition and specificity determinants of the human cbx chromodomains.
Kaustov L, Ouyang H, Amaya M, Lemak A, Nady N, Duan S, Wasney GA, Li Z, Vedadi M, Schapira M, Min J, Arrowsmith CH
J Biol Chem 286 521-9 (2011)
PMID: 21047797

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132085 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chromobox protein homolog 7 Chain: A
Molecule details ›
Chain: A
Length: 56 amino acids
Theoretical weight: 6.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95931 (Residues: 8-62; Coverage: 22%)
Gene name: CBX7
Sequence domains: Chromo (CHRromatin Organisation MOdifier) domain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Histone H3 Chain: B
Molecule details ›
Chain: B
Length: 15 amino acids
Theoretical weight: 1.52 KDa
Source organism: Xenopus laevis
Expression system: Not provided
UniProt:
  • Canonical: Q92133 (Residues: 20-34; Coverage: 11%)
Gene names: H3l, Hist1h3f, h3, h3.2a, h3c8, h3c8.S, h3r, hist1h3g, hist1h3g.L, hist2h3, hist2h3c

Ligands and Environments

No bound ligands
1 modified residue:
Ligand M3L 1 x M3L

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 84%
Refinement method: molecular dynamics
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

36 review citations

Roles of the Polycomb group proteins in stem cells and cancer.
Richly et al. (2011)
35 more

4 mentions without citation

Recognition and specificity determinants of the human cbx chromodomains.
Kaustov et al. (2011)
3 more