› 2l75

Solution NMR

Solution structure of CHD4-PHD2 in complex with H3K9me3

Released:
DOI: 10.2210/pdb2l75/pdb
PDB entry 2l75 coloured by chain, ensemble of 20 models, front view.
PDB entry 2l75 coloured by chain, ensemble of 20 models, side view.
PDB entry 2l75 coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Plant homeodomain (PHD) fingers of CHD4 are histone H3-binding modules with preference for unmodified H3K4 and methylated H3K9.
Mansfield RE, Musselman CA, Kwan AH, Oliver SS, Garske AL, Davrazou F, Denu JM, Kutateladze TG, Mackay JP
J Biol Chem 286 11779-91 (2011)
PMID: 21278251

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159406 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chromodomain-helicase-DNA-binding protein 4 Chain: A
Molecule details ›
Chain: A
Length: 61 amino acids
Theoretical weight: 6.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14839 (Residues: 446-501; Coverage: 3%)
Gene name: CHD4
Sequence domains: PHD-finger
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)
Histone H3.1 Chain: B
Molecule details ›
Chain: B
Length: 14 amino acids
Theoretical weight: 1.57 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 2-14; Coverage: 10%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand M3L 1 x M3L

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 85%
Refinement method: simulated annealing, molecular dynamics
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

33 review citations

Perceiving the epigenetic landscape through histone readers.
Musselman et al. (2012)
32 more

13 mentions without citation

Handpicking epigenetic marks with PHD fingers.
Musselman et al. (2011)
12 more