› 2lag

Solution NMR

Structure of the 44 kDa complex of interferon-alpha2 with the extracellular part of IFNAR2 obtained by 2D-double difference NOESY

Released:
DOI: 10.2210/pdb2lag/pdb
PDB entry 2lag coloured by chain, ensemble of 10 models, front view.
PDB entry 2lag coloured by chain, ensemble of 10 models, side view.
PDB entry 2lag coloured by chain, ensemble of 10 models, top view.
Source organism: Homo sapiens
Primary publication:
Observation of intermolecular interactions in large protein complexes by 2D-double difference nuclear Overhauser enhancement spectroscopy: application to the 44 kDa interferon-receptor complex.
Nudelman I, Akabayov SR, Scherf T, Anglister J
J Am Chem Soc 133 14755-64 (2011)
PMID: 21819146

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134393 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Interferon alpha/beta receptor 2 Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 24.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P48551 (Residues: 28-237; Coverage: 43%)
Gene names: IFNABR, IFNAR2, IFNARB
Sequence domains:
Structure domains: Immunoglobulins
Interferon alpha-2 Chain: A
Molecule details ›
Chain: A
Length: 165 amino acids
Theoretical weight: 19.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01563 (Residues: 24-188; Coverage: 100%)
Gene names: IFNA2, IFNA2A, IFNA2B, IFNA2C
Sequence domains: Interferon alpha/beta domain
Structure domains: Growth Hormone; Chain: A;

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 36%
Refinement method: simulated annealing
Expression system: Escherichia coli

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Citations

4 review citations

NMR approaches for structural analysis of multidomain proteins and complexes in solution.
Göbl et al. (2014)
3 more

2 mentions without citation

Observation of intermolecular interactions in large protein complexes by 2D-double difference nuclear Overhauser enhancement spectroscopy: application to the 44 kDa interferon-receptor complex.
Nudelman et al. (2011)
1 more