› 2len

Solution NMR

Solution structure of UCHL1 S18Y variant

Released:
DOI: 10.2210/pdb2len/pdb
PDB entry 2len coloured by chain, ensemble of 20 models, front view.
PDB entry 2len coloured by chain, ensemble of 20 models, side view.
PDB entry 2len coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Entry author: Tse H

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140823 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase isozyme L1 Chain: A
Molecule details ›
Chain: A
Length: 231 amino acids
Theoretical weight: 26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09936 (Residues: 1-223; Coverage: 100%)
Gene name: UCHL1
Sequence domains: Ubiquitin carboxyl-terminal hydrolase, family 1
Structure domains: Peptidase C12, ubiquitin carboxyl-terminal hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 79%
Refinement method: molecular dynamics
Expression system: Escherichia coli

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