› 2li5

Solution NMR

NMR structure of Atg8-Atg7C30 complex

Released:
DOI: 10.2210/pdb2li5/pdb
PDB entry 2li5 coloured by chain, ensemble of 19 models, front view.
PDB entry 2li5 coloured by chain, ensemble of 19 models, side view.
PDB entry 2li5 coloured by chain, ensemble of 19 models, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural basis of Atg8 activation by a homodimeric E1, Atg7.
Noda NN, Satoo K, Fujioka Y, Kumeta H, Ogura K, Nakatogawa H, Ohsumi Y, Inagaki F
Mol Cell 44 462-75 (2011)
PMID: 22055191

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153571 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Autophagy-related protein 8 Chain: A
Molecule details ›
Chain: A
Length: 117 amino acids
Theoretical weight: 13.6 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Aspergillus niger
UniProt:
  • Canonical: P38182 (Residues: 1-116; Coverage: 99%)
Gene names: APG8, ATG8, AUT7, CVT5, YBL0732, YBL078C
Sequence domains: Autophagy protein Atg8 ubiquitin like
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Ubiquitin-like modifier-activating enzyme ATG7 Chain: B
Molecule details ›
Chain: B
Length: 34 amino acids
Theoretical weight: 3.85 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P38862 (Residues: 601-630; Coverage: 5%)
Gene names: APG7, ATG7, CVT2, YHR171W

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 92%
Refinement method: simulated annealing, torsion angle dynamics
Expression systems:
  • Aspergillus niger
  • Escherichia coli

Quick links

Citations

33 review citations

The machinery of macroautophagy.
Feng et al. (2014)
32 more

4 mentions without citation

Comparative analyses of ubiquitin-like ATG8 and cysteine protease ATG4 autophagy genes in the plant lineage and cross-kingdom processing of ATG8 by ATG4.
Seo et al. (2016)
3 more