Function and Biology Details
Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
- not assigned
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137215 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bacterial RNA polymerase inhibitor
Molecule details ›
Chain: A
Length: 84 amino acids
Theoretical weight: 9.35 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
Sequence domains: RNA polymerase inhibitor
Structure domains: RNA polymerase inhibitor
Length: 84 amino acids
Theoretical weight: 9.35 KDa
Source organism: Escherichia phage T7
Expression system: Escherichia coli
UniProt:
- Canonical:
P03704 (Residues: 1-60; Coverage: 94%)
Sequence domains: RNA polymerase inhibitor
Structure domains: RNA polymerase inhibitor
DNA-directed RNA polymerase subunit beta'
Molecule details ›
Chain: B
Length: 84 amino acids
Theoretical weight: 9.36 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Length: 84 amino acids
Theoretical weight: 9.36 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
- Canonical: P0A8T7 (Residues: 1151-1213; Coverage: 5%)
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Chemical shift assignment:
85%
Refinement method:
simulated annealing
Expression system: Escherichia coli
