› 2mes

Solution NMR

Backbone 1H, 13C, 15N resonance assignments of calcium-bound calmodulin in complex with PSD95 N-terminal peptide

Released:
DOI: 10.2210/pdb2mes/pdb
PDB entry 2mes coloured by chain, ensemble of 10 models, front view.
PDB entry 2mes coloured by chain, ensemble of 10 models, side view.
PDB entry 2mes coloured by chain, ensemble of 10 models, top view.
Source organisms:
Primary publication:
Capping of the N-terminus of PSD-95 by calmodulin triggers its postsynaptic release.
Zhang Y, Matt L, Patriarchi T, Malik ZA, Chowdhury D, Park DK, Renieri A, Ames JB, Hell JW
EMBO J 33 1341-53 (2014)
PMID: 24705785

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160252 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.72 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
Disks large homolog 4 Chain: B
Molecule details ›
Chain: B
Length: 71 amino acids
Theoretical weight: 8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P78352 (Residues: 1-71; Coverage: 10%)
Gene names: DLG4, PSD95
Sequence domains: Polyubiquitination (PEST) N-terminal domain of MAGUK

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 53%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Genetic disorders associated with postnatal microcephaly.
Seltzer et al. (2014)
19 more

12 mentions without citation

The Origins and Roles of Methylthiolated Cytokinins: Evidence From Among Life Kingdoms.
Gibb et al. (2020)
11 more