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Solution NMR

Structural Characterization of Interactions between the Double-Stranded RNA-Binding Zinc Finger Protein JAZ and dsRNA

Released:
DOI: 10.2210/pdb2mkn/pdb
PDB entry 2mkn coloured by chain, front view.
PDB entry 2mkn coloured by chain, side view.
PDB entry 2mkn coloured by chain, top view.
Source organisms:
Primary publication:
Structural characterization of interactions between the double-stranded RNA-binding zinc finger protein JAZ and nucleic acids.
Burge RG, Martinez-Yamout MA, Dyson HJ, Wright PE
Biochemistry 53 1495-510 (2014)
PMID: 24521053

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193909 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Zinc finger protein 346 Chain: A
Molecule details ›
Chain: A
Length: 60 amino acids
Theoretical weight: 7.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UL40 (Residues: 168-227; Coverage: 20%)
Gene names: JAZ, ZNF346
Sequence domains: Zinc-finger of C2H2 type
Structure domains: Classic Zinc Finger
RNA (5'-R(*GP*CP*CP*GP*UP*GP*GP*UP*CP*UP*GP*GP*UP*GP*GP*CP*CP*GP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 19 nucleotides
Theoretical weight: 6.16 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA (5'-R(P*CP*CP*GP*GP*CP*CP*AP*CP*CP*AP*GP*AP*CP*CP*AP*CP*GP*GP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 19 nucleotides
Theoretical weight: 6.05 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 13%
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

NMR solution structure determination of large RNA-protein complexes.
Yadav et al. (2016)
7 other articles

3 mentions without citation

Structural characterization of interactions between the double-stranded RNA-binding zinc finger protein JAZ and nucleic acids.
Burge et al. (2014)
2 more