› 2mv7

Solution NMR

Solution NMR structure of DOT1L in complex with AF9 (DOT1L-AF9)

Released:
DOI: 10.2210/pdb2mv7/pdb
PDB entry 2mv7 coloured by chain, ensemble of 10 models, front view.
PDB entry 2mv7 coloured by chain, ensemble of 10 models, side view.
PDB entry 2mv7 coloured by chain, ensemble of 10 models, top view.
Source organism: Homo sapiens
Primary publication:
Degree of recruitment of DOT1L to MLL-AF9 defines level of H3K79 Di- and tri-methylation on target genes and transformation potential.
Kuntimaddi A, Achille NJ, Thorpe J, Lokken AA, Singh R, Hemenway CS, Adli M, Zeleznik-Le NJ, Bushweller JH
Cell Rep 11 808-20 (2015)
PMID: 25921540

Function and Biology Details

Reaction catalysed: 
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(79) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(79)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154678 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein AF-9 Chain: A
Molecule details ›
Chain: A
Length: 69 amino acids
Theoretical weight: 8.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P42568 (Residues: 500-568; Coverage: 12%)
Gene names: AF9, MLLT3, YEATS3
Sequence domains: ANC1 homology domain (AHD)
Histone-lysine N-methyltransferase, H3 lysine-79 specific Chain: B
Molecule details ›
Chain: B
Length: 25 amino acids
Theoretical weight: 2.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TEK3 (Residues: 877-900; Coverage: 2%)
Gene names: DOT1L, KIAA1814, KMT4

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 87%
Refinement method: simulated annealing, SIMULATED ANNEALING, SIMULATED ANNEALING
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Targeting transcription factors in cancer - from undruggable to reality.
Bushweller JH. (2019)
20 more

9 mentions without citation

Activation and regulation of H2B-Ubiquitin-dependent histone methyltransferases.
Worden et al. (2019)
8 more