› 2n5b

Solution NMR

Structures of the OXIDIZED state of the mutant D24A of yeast thioredoxin 1

Released:
DOI: 10.2210/pdb2n5b/pdb
PDB entry 2n5b coloured by chain, ensemble of 20 models, front view.
PDB entry 2n5b coloured by chain, ensemble of 20 models, side view.
PDB entry 2n5b coloured by chain, ensemble of 20 models, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structures of the reduced and oxidized state of the mutant D24A of yeast thioredoxin 1: insights into the mechanism for the closing of the water cavity.
Iqbal A, Moraes AH, Valente AP, Almeida FCL
J Biomol NMR 63 417-423 (2015)
PMID: 26482062

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149546 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin-1 Chain: A
Molecule details ›
Chain: A
Length: 103 amino acids
Theoretical weight: 11.2 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22217 (Residues: 1-103; Coverage: 100%)
Gene names: TRX1, TRX2, YLR043C
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 87%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Polyamine-Based Thiols in Trypanosomatids: Evolution, Protein Structural Adaptations, and Biological Functions.
Manta et al. (2018)

1 mention without citation

Using NMR Chemical Shifts and Cryo-EM Density Restraints in Iterative Rosetta-MD Protein Structure Refinement.
Leelananda et al. (2020)