› 2n82

Solution NMR

solution structure of the complex of microRNA 20b pre-element with Rbfox RRM

Released:
DOI: 10.2210/pdb2n82/pdb
PDB entry 2n82 coloured by chain, ensemble of 20 models, front view.
PDB entry 2n82 coloured by chain, ensemble of 20 models, side view.
PDB entry 2n82 coloured by chain, ensemble of 20 models, top view.
Source organisms:
Primary publication:
Rbfox proteins regulate microRNA biogenesis by sequence-specific binding to their precursors and target downstream Dicer.
Chen Y, Zubovic L, Yang F, Godin K, Pavelitz T, Castellanos J, Macchi P, Varani G
Nucleic Acids Res 44 4381-95 (2016)
PMID: 27001519

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192651 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RNA binding protein fox-1 homolog 1 Chain: B
Molecule details ›
Chain: B
Length: 104 amino acids
Theoretical weight: 11.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NWB1 (Residues: 109-208; Coverage: 25%)
Gene names: A2BP, A2BP1, FOX1, HRNBP1, RBFOX1
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
RNA (5'-R(*GP*GP*UP*AP*GP*UP*UP*UP*UP*GP*GP*CP*AP*UP*GP*AP*CP*UP*CP*UP*AP*CP*C)-3') Chain: A
Molecule details ›
Chain: A
Length: 23 nucleotides
Theoretical weight: 7.32 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 80%
Refinement method: restrained simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

15 review citations

Regulation of microRNA biogenesis and its crosstalk with other cellular pathways.
Treiber et al. (2019)
14 more

6 mentions without citation

Rbfox proteins regulate microRNA biogenesis by sequence-specific binding to their precursors and target downstream Dicer.
Chen et al. (2016)
5 more