› 2nbq

Solution NMR

NMR Structure of the C-Terminal Domain of human APOBEC3B

Released:
DOI: 10.2210/pdb2nbq/pdb
PDB entry 2nbq coloured by chain, ensemble of 30 models, front view.
PDB entry 2nbq coloured by chain, ensemble of 30 models, side view.
PDB entry 2nbq coloured by chain, ensemble of 30 models, top view.
Source organism: Homo sapiens
Primary publication:
Nuclear Magnetic Resonance Structure of the APOBEC3B Catalytic Domain: Structural Basis for Substrate Binding and DNA Deaminase Activity.
Byeon IJ, Byeon CH, Wu T, Mitra M, Singer D, Levin JG, Gronenborn AM
Biochemistry 55 2944-59 (2016)
PMID: 27163633

Function and Biology Details

Reaction catalysed: 
Cytosine in single-stranded DNA + H(2)O = uracil in single-stranded DNA + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193726 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA dC->dU-editing enzyme APOBEC-3B Chain: A
Molecule details ›
Chain: A
Length: 205 amino acids
Theoretical weight: 24.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UH17 (Residues: 187-382; Coverage: 51%)
Gene name: APOBEC3B
Sequence domains: APOBEC-like C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 67%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

APOBEC3s: DNA-editing human cytidine deaminases.
Silvas et al. (2019)
6 more

4 mentions without citation

A Novel Regulator of Activation-Induced Cytidine Deaminase/APOBECs in Immunity and Cancer: Schrödinger's CATalytic Pocket.
King et al. (2017)
3 more