› 2nbv

Solution NMR

Solution structure of the Rpn13 Pru domain engaging the hPLIC2 UBL domain

Released:
DOI: 10.2210/pdb2nbv/pdb
PDB entry 2nbv coloured by chain, ensemble of 20 models, front view.
PDB entry 2nbv coloured by chain, ensemble of 20 models, side view.
PDB entry 2nbv coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Structures of Rpn1 T1:Rad23 and hRpn13:hPLIC2 Reveal Distinct Binding Mechanisms between Substrate Receptors and Shuttle Factors of the Proteasome.
Chen X, Randles L, Shi K, Tarasov SG, Aihara H, Walters KJ
Structure 24 1257-1270 (2016)
PMID: 27396824

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172451 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proteasomal ubiquitin receptor ADRM1 Chain: A
Molecule details ›
Chain: A
Length: 150 amino acids
Theoretical weight: 16.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16186 (Residues: 1-150; Coverage: 37%)
Gene names: ADRM1, GP110
Sequence domains: Proteasome complex subunit Rpn13, Pru domain
Structure domains: Proteasomal ubiquitin receptor Rpn13/ADRM1
Ubiquilin-2 Chain: B
Molecule details ›
Chain: B
Length: 78 amino acids
Theoretical weight: 8.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UHD9 (Residues: 26-103; Coverage: 13%)
Gene names: HRIHFB2157, N4BP4, PLIC2, UBQLN2
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 47%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Meddling with Fate: The Proteasomal Deubiquitinating Enzymes.
de Poot et al. (2017)
14 more

3 mentions without citation

Structure and Function of the 26S Proteasome.
Bard et al. (2018)
2 more