› 2ndf

Solution NMR

Solution NMR structures of AF9 yeats domain in complex with histon H3 acetylation at K18

Released:
DOI: 10.2210/pdb2ndf/pdb
PDB entry 2ndf coloured by chain, ensemble of 20 models, front view.
PDB entry 2ndf coloured by chain, ensemble of 20 models, side view.
PDB entry 2ndf coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Structural Insights into Histone Crotonyl-Lysine Recognition by the AF9 YEATS Domain.
Zhang Q, Zeng L, Zhao C, Ju Y, Konuma T, Zhou MM
Structure 24 1606-12 (2016)
PMID: 27545619

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154673 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein AF-9 Chain: A
Molecule details ›
Chain: A
Length: 141 amino acids
Theoretical weight: 16.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42568 (Residues: 1-138; Coverage: 24%)
Gene names: AF9, MLLT3, YEATS3
Sequence domains: YEATS family
Structure domains: YEATS domain
Histone H3.1 Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.37 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 13-25; Coverage: 10%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

No bound ligands
1 modified residue:
Ligand ALY 1 x ALY

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 85%
Refinement method: distance geometry, simulated annealing, torsion angle dynamics
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

13 review citations

Metabolic regulation of gene expression through histone acylations.
Sabari et al. (2017)
12 more

2 mentions without citation

Structural Insights into Histone Crotonyl-Lysine Recognition by the AF9 YEATS Domain.
Zhang et al. (2016)
1 more