› 2nlx

X-ray diffraction
2.7Å resolution

Crystal structure of the apo E. coli xylulose kinase

Released:
DOI: 10.2210/pdb2nlx/pdb
PDB entry 2nlx coloured by chain, front view.
PDB entry 2nlx coloured by chain, side view.
PDB entry 2nlx coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural and kinetic studies of induced fit in xylulose kinase from Escherichia coli.
Di Luccio E, Petschacher B, Voegtli J, Chou HT, Stahlberg H, Nidetzky B, Wilson DK
J Mol Biol 365 783-98 (2007)
PMID: 17123542

Function and Biology Details

Reaction catalysed: 
ATP + D-xylulose = ADP + D-xylulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140552 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xylulose kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 484 amino acids
Theoretical weight: 52.67 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P09099 (Residues: 1-484; Coverage: 100%)
Gene names: JW3536, b3564, xylB
Sequence domains:
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 67.78Å b: 112.02Å c: 138.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.254
Expression system: Escherichia coli

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Citations

2 review citations

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
1 more

3 mentions without citation

The FGGY carbohydrate kinase family: insights into the evolution of functional specificities.
Zhang et al. (2011)
2 more