› 2npp

X-ray diffraction
3.3Å resolution

Structure of the Protein Phosphatase 2A Holoenzyme

Released:
DOI: 10.2210/pdb2npp/pdb
PDB entry 2npp coloured by chain, front view.
PDB entry 2npp coloured by chain, side view.
PDB entry 2npp coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the protein phosphatase 2A holoenzyme.
Xu Y, Xing Y, Chen Y, Chao Y, Lin Z, Fan E, Yu JW, Strack S, Jeffrey PD, Shi Y
Cell 127 1239-51 (2006)
PMID: 17174897

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-151657 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Chains: A, D
Molecule details ›
Chains: A, D
Length: 589 amino acids
Theoretical weight: 65.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30153 (Residues: 1-589; Coverage: 100%)
Gene name: PPP2R1A
Sequence domains: HEAT repeat
Structure domains: Leucine-rich Repeat Variant
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform Chains: B, E
Molecule details ›
Chains: B, E
Length: 449 amino acids
Theoretical weight: 52.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13362 (Residues: 1-442; Coverage: 84%)
Gene names: KIAA0044, PPP2R5C
Sequence domains: Protein phosphatase 2A regulatory B subunit (B56 family)
Structure domains: Leucine-rich Repeat Variant
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Chains: C, F
Molecule details ›
Chains: C, F
Length: 309 amino acids
Theoretical weight: 35.64 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P67775 (Residues: 1-309; Coverage: 100%)
Gene name: PPP2CA
Sequence domains: Calcineurin-like phosphoesterase
Structure domains: Purple Acid Phosphatase; chain A, domain 2
microcystin LR Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 7 amino acids
Theoretical weight: 1.01 KDa
Source organism: Cyanobacteriota
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MN 4 x MN
3 modified residues:
Ligand DAL 2 x DAL
Ligand ACB 2 x ACB
Ligand FGA 2 x FGA

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 109.26Å b: 159.05Å c: 269.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.285 0.255 0.299
Expression systems:
  • Escherichia coli BL21(DE3)
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

59 review citations

Serine/threonine phosphatases: mechanism through structure.
Shi Y. (2009)
58 more

26 mentions without citation

Phosphatases in Mitosis: Roles and Regulation.
Moura et al. (2019)
25 more